The Bio-Men first appeared in the movie Dragon Ball Z: The World's Strongest, first seen when they launched a surprise attack on Gohan, who they outnumbered and quickly overwhelmed. Piccolo managed to overpower the Bio-Men even killing one with a Mouth Energy Wave, but was subdued by Doctors Wheelo and Kochin's other minions. The Bio-Men later fought against Master Roshi, though Roshi demonstrated that he was more than a match for them. After Roshi was distracted by Dr. Kochin, the Bio-Men got a hold of Bulma threatening to kill her if Roshi did not accompany him to Dr. Wheelo.
The Bio-men were strong enough to harm Gohan when they ambushed him however the combined might of four them together were easily beaten by Master Roshi.
- Flight – The ability to fly using ki. Used in Dragon Ball Heroes.
- Ki Blast – The most basic form of energy wave. Used in Dragon Ball Heroes.
- Biobomb – Bio-Man's super attack in the arcade game Dragon Ball Heroes. Bio-Man leaps in front of the opponent and explodes.
Bio-Men made their video game debut in the Japanese-only arcade game Dragon Ball Heroes.
- Japanese: Hitoshi Bifu, Kōzō Shioya
- Ocean dub: Alec Willows
- AB Groupe dub: Doug Rand
- Funimation dub: Chris Cason, John Burgmeier
- Brazilian Portuguese dub: Sérgio Rufino
- Bio-Men bare a striking resemblance to Saibamen, though their bluish-gray skin and different facial features make them easy to distinguish from the Saibamen. Saibamen are also less verbal and appear to be stronger than Bio-Men, as Master Roshi (who was far weaker than a Saibaman) was able to defeat several Bio-Men at once relatively easily. In addition, one of them is killed by Piccolo in a similar manner to the last Saibaman used by Nappa on Earth.
- In the video game Dragon Ball Z: Attack of the Saiyans, enemies called Experiment #3, Experiment #7, and Experiment #9 look similar to Bio-Men.